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Gene Cloning

Gene Cloning PDF
Author: David M. Glover
Publisher: Springer
ISBN: 1489932461
Size: 44.42 MB
Format: PDF, ePub
Category : Medical
Languages : un
Pages : 222
View: 4676

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Gene Cloning

by David M. Glover, Gene Cloning Books available in PDF, EPUB, Mobi Format. Download Gene Cloning books, This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA. It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size. I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion. Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop ment ofother vectors has certainly not stood still over the past four years. This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.




Molecular Cloning

Molecular Cloning PDF
Author: Joseph Sambrook
Publisher:
ISBN: 9780879695767
Size: 20.56 MB
Format: PDF, Kindle
Category : Science
Languages : un
Pages : 2344
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Molecular Cloning

by Joseph Sambrook, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books, The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.




Guide To Molecular Cloning Techniques

Guide to Molecular Cloning Techniques PDF
Author: Shelby L. Berger
Publisher:
ISBN: 9780121817756
Size: 32.89 MB
Format: PDF, ePub
Category : Science
Languages : un
Pages : 812
View: 3161

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Guide To Molecular Cloning Techniques

by Shelby L. Berger, Guide To Molecular Cloning Techniques Books available in PDF, EPUB, Mobi Format. Download Guide To Molecular Cloning Techniques books, Guide to Molecular Cloning Techniques is a self-contained, state-of-the-art manual designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Key Features * Comprehensive protocols compiled specifically for this volume * Among the topics covered * Genomic cloning * Preparation and characterization of mRNA * cDNA cloning * Screening libraries * Identification and characterization of clones * Methods written by 87 experts * Editors' notes, overviews, and extensive cross-referencing * Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility * A problem-solving quantitative approach * Emphasis on strategies for choosing alternative methods




Vectors

Vectors PDF
Author: Raymond L. Rodriguez
Publisher: Butterworth-Heinemann
ISBN: 1483103080
Size: 70.84 MB
Format: PDF
Category : Science
Languages : un
Pages : 592
View: 2964

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Vectors

by Raymond L. Rodriguez, Vectors Books available in PDF, EPUB, Mobi Format. Download Vectors books, Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.




Molecular Cloning

Molecular Cloning PDF
Author: Michael Richard Green
Publisher:
ISBN:
Size: 64.17 MB
Format: PDF, ePub
Category : Molecular cloning
Languages : un
Pages : 1936
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Molecular Cloning

by Michael Richard Green, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books, Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. [...] The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description.




Molecular Cloning

Molecular Cloning PDF
Author: Gregory G. Brown
Publisher: BoD – Books on Demand
ISBN: 9533073985
Size: 71.16 MB
Format: PDF, ePub, Mobi
Category : Medical
Languages : en
Pages : 338
View: 2716

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Molecular Cloning

by Gregory G. Brown, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books, The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.




Molecular Cloning And Gene Regulation In Bacilli

Molecular Cloning and Gene Regulation in Bacilli PDF
Author: A Ganesan
Publisher: Elsevier
ISBN: 0323153097
Size: 32.69 MB
Format: PDF, Kindle
Category : Nature
Languages : en
Pages : 382
View: 269

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Molecular Cloning And Gene Regulation In Bacilli

by A Ganesan, Molecular Cloning And Gene Regulation In Bacilli Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning And Gene Regulation In Bacilli books, Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation. Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid vector of large DNA segments, and the mating system of bacilli. Molecular cloning session covers complementation system and dominance analyses in Bacillus, genetic fusion of Escherichia coli lac genes to a Bacillus subtilis promoter, and DNA cloning of B. subtilis. It also describes the construction of trimethoprim resistant B. subtilis plasmid and expression of E. coli trp genes cloned in B. subtilis. Session III encompasses chapters that discuss protein secretion by bacilli; regulation of alpha-amylase production in B. subtilis; entomocidal toxin translation of B. thuringiensis; and expression of crystal protein, heterologous, and eukaryotic genes in bacilli. Session IV focuses on various aspects of DNA metabolism of bacilli, such as the interaction of bacterial chromosome with cell membrane; plasmid DNA in competent cells and protoplasts of B. subtilis; analysis of peptides synthesized by B. subtilis mutants; and DNA repair, uptake, restriction, modification, and recombination. The final session examines species-specific translation, control of gene expression and replications in plasmids, development of expression-vector in B. subtilis, and regulatory modifications of RNA polymerase. Each chapter is presented in an experimental manner, consisting of a summary of the study, materials and methods, results, as well as references.




The Condensed Protocols From Molecular Cloning A Laboratory Manual

The Condensed Protocols from Molecular Cloning   a Laboratory Manual PDF
Author: Joseph Sambrook
Publisher: CSHL Press
ISBN: 0879697717
Size: 20.69 MB
Format: PDF, ePub
Category : Science
Languages : un
Pages : 800
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The Condensed Protocols From Molecular Cloning A Laboratory Manual

by Joseph Sambrook, The Condensed Protocols From Molecular Cloning A Laboratory Manual Books available in PDF, EPUB, Mobi Format. Download The Condensed Protocols From Molecular Cloning A Laboratory Manual books, The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.




Molecular Cloning Of Hormone Genes

Molecular Cloning of Hormone Genes PDF
Author: Joel F. Habener
Publisher: Springer Science & Business Media
ISBN: 1461248248
Size: 36.72 MB
Format: PDF, ePub, Docs
Category : Science
Languages : un
Pages : 442
View: 7241

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Molecular Cloning Of Hormone Genes

by Joel F. Habener, Molecular Cloning Of Hormone Genes Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning Of Hormone Genes books, The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry onic development. During the past ten years, refinements in the techniques of recombinant DNA technology have resulted in the cloning of genes encoding approximately 50 different hormonal and regulatory peptides, including those in which the peptides themselves and the mRNAs encoding the peptides are present in only trace amounts in the tissues of origin. In addition to provid ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors. The encoding of multiple peptides in a single mono cistronic mRNA appears to be a genetic mechanism for the gener ation of biologic diversification without requiring amplification of gene sequences. Two of the objectives in the assembly of this book are to pre sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related regulatory peptides, and to provide an account of the various ap proaches that have been used to identify and select the cloned genes encoding these polypeptides. The contents of the two in troductory chapters are intended to provide the reader with a brief background of the approaches to gene cloning and the struc ture and expression of hormone-encoding genes.




Molecular Biology Techniques

Molecular Biology Techniques PDF
Author: Sue Carson
Publisher: Academic Press
ISBN: 0128157755
Size: 34.71 MB
Format: PDF, ePub
Category : Science
Languages : un
Pages : 294
View: 3499

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Molecular Biology Techniques

by Sue Carson, Molecular Biology Techniques Books available in PDF, EPUB, Mobi Format. Download Molecular Biology Techniques books, Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions