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Molecular Cloning

Molecular Cloning PDF
Author: Michael Richard Green
Publisher:
ISBN:
Size: 37.90 MB
Format: PDF, Mobi
Category : Molecular cloning
Languages : en
Pages : 1936
View: 4562

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Molecular Cloning

by Michael Richard Green, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books, Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. [...] The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description.




Guide To Molecular Cloning Techniques

Guide to Molecular Cloning Techniques PDF
Author: Shelby L. Berger
Publisher:
ISBN: 9780121817756
Size: 38.31 MB
Format: PDF
Category : Science
Languages : en
Pages : 812
View: 4666

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Guide To Molecular Cloning Techniques

by Shelby L. Berger, Guide To Molecular Cloning Techniques Books available in PDF, EPUB, Mobi Format. Download Guide To Molecular Cloning Techniques books, Guide to Molecular Cloning Techniques is a self-contained, state-of-the-art manual designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Key Features * Comprehensive protocols compiled specifically for this volume * Among the topics covered * Genomic cloning * Preparation and characterization of mRNA * cDNA cloning * Screening libraries * Identification and characterization of clones * Methods written by 87 experts * Editors' notes, overviews, and extensive cross-referencing * Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility * A problem-solving quantitative approach * Emphasis on strategies for choosing alternative methods




Molecular Cloning

Molecular Cloning PDF
Author: Joseph Sambrook
Publisher: CSHL Press
ISBN: 9780879695774
Size: 21.33 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages :
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Molecular Cloning

by Joseph Sambrook, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books, The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.




Gene Cloning

Gene Cloning PDF
Author: David M. Glover
Publisher: Springer
ISBN: 1489932461
Size: 19.47 MB
Format: PDF, ePub, Mobi
Category : Medical
Languages : en
Pages : 222
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Gene Cloning

by David M. Glover, Gene Cloning Books available in PDF, EPUB, Mobi Format. Download Gene Cloning books, This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA. It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size. I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion. Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop ment ofother vectors has certainly not stood still over the past four years. This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.




Molecular Cloning

Molecular Cloning PDF
Author: Joseph Sambrook
Publisher:
ISBN:
Size: 37.71 MB
Format: PDF, ePub, Docs
Category : Eukaryotic cells
Languages : en
Pages : 676
View: 6800

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Molecular Cloning

by Joseph Sambrook, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books,




The Condensed Protocols From Molecular Cloning A Laboratory Manual

The Condensed Protocols from Molecular Cloning   a Laboratory Manual PDF
Author: Joseph Sambrook
Publisher: CSHL Press
ISBN: 0879697717
Size: 11.95 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 800
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The Condensed Protocols From Molecular Cloning A Laboratory Manual

by Joseph Sambrook, The Condensed Protocols From Molecular Cloning A Laboratory Manual Books available in PDF, EPUB, Mobi Format. Download The Condensed Protocols From Molecular Cloning A Laboratory Manual books, The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.




Molecular Cloning

Molecular Cloning PDF
Author: Tom Maniatis
Publisher:
ISBN:
Size: 21.32 MB
Format: PDF, ePub, Docs
Category : Eukaryotic cells
Languages : en
Pages : 545
View: 6604

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Molecular Cloning

by Tom Maniatis, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books,




Fundamental Molecular Biology

Fundamental Molecular Biology PDF
Author: Lizabeth A. Allison
Publisher: John Wiley & Sons
ISBN: 1119156297
Size: 71.69 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 816
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Fundamental Molecular Biology

by Lizabeth A. Allison, Fundamental Molecular Biology Books available in PDF, EPUB, Mobi Format. Download Fundamental Molecular Biology books, Fundamental Molecular Biology Discover a focused and up to date exploration of foundational and core concepts in molecular biology The newly revised Third Edition of Fundamental Molecular Biology delivers a selective and precise treatment of essential topics in molecular biology perfect for allowing students to develop an accurate understanding of the applications of the field. The book applies the process of discovery-observations, questions, experimental designs, results, and conclusions-with an emphasis on the language of molecular biology. Readers will easily focus on the key ideas they need to succeed in any introductory molecular biology course. Fundamental Molecular Biology provides students with the most up to date techniques and research used by molecular biologists today. Readers of the book will have the support and resources they need to develop a concrete understanding of core and foundational concepts of molecular biology, without being distracted by outdated or peripheral material. Readers will also benefit from the inclusion of: A thorough introduction to and comparison of eukaryotic and prokaryotic organisms illustrating the variation of cellular processes across organisms Tool boxes exploring up to date experimental methods and techniques used by molecular biologists Focus boxes providing detailed treatment of topics that delve further into experimental strategies Disease boxes placing complex regulatory pathways in their relevant context and illustrating key principles of molecular biology Perfect for instructors and professors of introductory molecular biology courses, Fundamental Molecular Biology will also earn a place in the libraries of anyone seeking to improve their understanding of molecular biology with an insightful and well-grounded treatment of the core principles of the subject.




Molecular Biology

Molecular Biology PDF
Author: David P. Clark
Publisher: Elsevier
ISBN: 0123785901
Size: 30.85 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 784
View: 658

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Molecular Biology

by David P. Clark, Molecular Biology Books available in PDF, EPUB, Mobi Format. Download Molecular Biology books, Molecular Biology: Academic Cell Update provides an introduction to the fundamental concepts of molecular biology and its applications. It deliberately covers a broad range of topics to show that molecular biology is applicable to human medicine and health, as well as veterinary medicine, evolution, agriculture, and other areas. The present Update includes journal specific images and test bank. It also offers vocabulary flashcards. The book begins by defining some basic concepts in genetics such as biochemical pathways, phenotypes and genotypes, chromosomes, and alleles. It explains the characteristics of cells and organisms, DNA, RNA, and proteins. It also describes genetic processes such as transcription, recombination and repair, regulation, and mutations. The chapters on viruses and bacteria discuss their life cycle, diversity, reproduction, and gene transfer. Later chapters cover topics such as molecular evolution; the isolation, purification, detection, and hybridization of DNA; basic molecular cloning techniques; proteomics; and processes such as the polymerase chain reaction, DNA sequencing, and gene expression screening. Up to date description of genetic engineering, genomics, and related areas Basic concepts followed by more detailed, specific applications Hundreds of color illustrations enhance key topics and concepts Covers medical, agricultural, and social aspects of molecular biology Organized pedagogy includes running glossaries and keynotes (mini-summaries) to hasten comprehension




Molecular Cloning

Molecular Cloning PDF
Author: Gregory G. Brown
Publisher: BoD – Books on Demand
ISBN: 9533073985
Size: 42.63 MB
Format: PDF
Category : Medical
Languages : en
Pages : 338
View: 2291

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Molecular Cloning

by Gregory G. Brown, Molecular Cloning Books available in PDF, EPUB, Mobi Format. Download Molecular Cloning books, The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.